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Paola Cremonesi

Researcher

 

Phone: +39 0371 4662508

Fax: +39 0371 4662501

Address: IBBA-CNR, U.O.S. di Lodi, Via Einstein, 26900 Lodi

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Education and Professional Experience

 

2010-present: Permanent position as Researcher at the Italian National Research Council, Institute of Agricultural Biology and Biotechnology (IBBA-CNR), Lodi.

2009-2010: Temporary position as researcher at Institute of Agricultural Biology and Biotechnology (IBBA-CNR). Field of activity: SNP bovine genotyping (genes involved in milk quality and production, milk fat biosynthesis, genes having DNA-binding and transcriptional activation ability, and hormonal, receptor or transport functions). This work is supported by Accordo Quadro CNR – Regione Lombardia (http://ribitinnova.ibba.cnr.it/).

2007-2008: Fellowship at Italian National Research Council (IBBA-CNR), Supervisor: Dott.ssa Bianca Castiglioni. Field of activity: development of a microarray for the detection of the main pathogens responsible for subclinical mastitis in bovine related to the “SAFEMILK” project (project n°962 “Diagnosi precoce di mastiti subcliniche per un miglioramento quali-quantitativo delle produzioni lattiero-casearie”).

2005-2006: Fellowship at University of Milan, Department of Biomedical Sciences and Technology. Supervisor: Prof. Massimo Luzzana. Field of activity: molecular and microbiological targets for dairy-food certification.

2001-2004: Ph.D. in Molecular Medicine (Genomics, Proteomics and Correlated Technologies) at the University of Milan, Department of Biomedical Sciences and Technology. Supervisor: Prof. Massimo Luzzana. Field of activity: development of techniques for the detection, directly from milk samples, of the main pathogens (for example Staphylococcus aureus) known to cause mastitis in bovine, ovine and caprine and/or responsible for foodborne intoxication.

1998-2001: Staff scientist for Menarini Diagnostics (Florence, Italy). Field of activity: development of a subcutaneous glucose sensor for the on-line monitoring of the glycaemia in diabetic patients.

From 1998: Licensing of professional biologist.

1997: Degree in Biology at the University of Milan.

 

Keywords

Plant and Animal Biodiversity, Gene expression regulation, Traceability and safety of feed and food products

Molecular biology, Molecular diagnostics of feed and foods, Biostatistics and bioinformatics

 

Ongoing projects 

2013-2015: WorkPackage 6. Biodiversita’ degli agroecosistemi alpini in the framework of the Agreement Regione Lombardia - CNR, project FilAgro "Strategie innovative e sostenibili per la filiera agroalimentare".

2013-2015: WorkPackage 7. Allevamento e gestione degli animali, analisi del trascrittoma della ghiandola mammaria e del metagenoma ruminale, in the framework of the Agreement Regione Lombardia - CNR, project FilAgro "Strategie innovative e sostenibili per la filiera agroalimentare".

2013-2015: WorkPackage 3.2 Fisiologia della riproduzione, Task 3.2.3 Fertilità degli spermatozoi, within the project GenHome.

2011-2014: project: "Genetic and functional characterization of autochthonous pig breeds in Southern Italy for the valorization of local products and for the conservation of biodiversity" within the national project: “Integrated Knowledge for Sustainability and Innovation of “Made in Italy” in the Agroalimentary System (CISIA)”.

2011-2014: project "MASTFIELD - Applicazione di sistemi molecolari innovativi per il controllo in campo delle mastiti bovine”, funded by Regione Lombardia.

2010-2011: European project "Interreg Innovation for Welfare: FoBoS - Sharing Molecular techniques for Food Borne Pathogen Detection” Project.

2009-2010: WorkPackage 4.1. High-throughput genotyping tools for diagnostics and selection in Italian cattle breeds, within the project “Accordo Quadro CNR - Regione Lombardia: Biological resources and innovative technologies for the sustainable development of the Agroalimentary System”.

2006-2008: project "SAFEMILK: Diagnosi precoce di mastiti subcliniche per un miglioramento quali-quantitativo delle produzioni lattiero-casearie”, funded by Regione Lombardia.

2006-2007: project F.I.R.B.: “Identificazione di alterazioni molecolari per la diagnosi e la prognosi del carcinoma renale con tecniche genomiche e proteomiche N. RBNE01HCKF-002”.

 

Selected Publications

  • Cremonesi P, Pisani LF, Lecchi C, Ceciliani F, Martino P, Bonastre AS, Karus A, Balzaretti C, Castiglioni B. (2014). Development of 23 individual TaqMan(®) real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions. Food Microbiology 43:35-40.

  • Böhme K, Cremonesi P, Severgnini M, Villa TG, Fernández-No IC, Barros-Velázquez J, Castiglioni B, Calo-Mata P. (2014). Detection of food spoilage and pathogenic bacteria based on ligation detection reaction coupled to flow-through hybridization on membranes. Biomed Research International 2014;2014:156323. doi: 10.1155/2014/156323. Epub 2014 Apr 10. PubMed PMID: 24818128.

  • Luzzago C, Locatelli C, Franco A, Scaccabarozzi L, Gualdi V, Viganò R, Sironi G, Besozzi M, Castiglioni B, Lanfranchi P, Cremonesi P, Battisti A. (2014). Clonal diversity, virulence-associated genes and antimicrobial resistance profile of Staphylococcus aureus isolates from nasal cavities and soft tissue infections in wild ruminants in Italian Alps. Veterinary Microbiology 170(1-2):157-61.

  • Modesto P, Peletto S, Pisoni G, Cremonesi P, Castiglioni B, Colussi S, Caramelli M, Bronzo V, Moroni P, Acutis PL. (2013). Evaluation of internal reference genes for quantitative expression analysis by real-time reverse transcription-PCR in somatic cells from goat milk. Journal of Dairy Science 96(12):7932-44. doi: 10.3168/jds.2012-6383.

  • Morandi S, Cremonesi P, Silvetti T, Brasca M. (2013). Technological characterisation, antibiotic susceptibility and antimicrobial activity of wild-type Leuconostoc strains isolated from North Italian traditional cheeses. Journal of Dairy Research 80(4):457-66. doi: 10.1017/S0022029913000447.

  • Cremonesi P, Zottola T, Locatelli C, Pollera C, Castiglioni B, Scaccabarozzi L, Moroni P. (2013). Identification of virulence factors in 16S-23S rRNA intergenic spacer genotyped Staphylococcus aureus isolated from water buffaloes and small ruminants. Journal of Dairy Science 96(12):7666-7674. doi: 10.3168/jds.2013-6917.

  • Cremonesi P, Chessa S, Castiglioni B. (2013). Genome sequence and analysis of Lactobacillus helveticus. Frontiers Microbiology 3:435. doi:10.3389/fmicb.2012.00435.

  • Aparicio-Fabre R, Guillén G, Loredo M, Arellano J, Valdés-López O, Ramírez M, Iñiguez LP, Panzeri D, Castiglioni B, Cremonesi P, Strozzi F, Stella A, Girard L,Sparvoli F, Hernández G. (2013). Common bean (Phaseolus vulgaris L.) PvTIFY orchestrates global changes in transcript profile response to jasmonate and phosphorus deficiency. BMC Plant Biology 13:26. doi: 10.1186/1471-2229-13-26.

  • Ramírez M, Guillén G, Fuentes SI, Iñiguez LP, Aparicio-Fabre R, Zamorano-Sánchez D, Encarnación-Guevara S, Panzeri D, Castiglioni B, Cremonesi P, Strozzi F, Stella A, Girard L, Sparvoli F, Hernández G. (2013). Transcript profiling of common bean nodules subjected to oxidative stress. Physiology Plant 149(3):389-407. doi: 10.1111/ppl.12040.

  • Cremonesi P, Capoferri R, Pisoni G, Del Corvo M, Strozzi F, Rupp R, Caillat H, Modesto P, Moroni P, Williams JL, Castiglioni B, Stella A. (2012). Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells. BMC Genomics 13(1):540. PubMed PMID: 23046560.

  • Cremonesi P, Pozzi F, Ricchi M, Castiglioni B, Luini M, Chessa S. (2012). Technical note: Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism. Journal of Dairy Science doi:pii:S0022-0302(12)00698-4. 10.3168/jds.2012-5785.

  • Cremonesi P, Vanoni L, Silvetti T, Morandi S, Brasca M. (2012). Identification of Clostridium beijerinckii, Cl. butyricum, Cl. sporogenes, Cl. tyrobutyricum isolated from silage, raw milk and hard cheese by a multiplex PCR assay. Journal of Dairy Research 79(3):318-323.

  • Severgnini M, Cremonesi P, Consolandi C, De Bellis G, Castiglioni B. (2011). Advances in DNA Microarray Technology for the Detection of Foodborne Pathogens. Food and Bioprocess Technology, pp. 1-18.

  • Cremonesi P, Vanoni L, Morandi S, Silvetti T, Castiglioni B, Brasca M. (2011). Development of a pentaplex PCR assay for the simultaneous detection of Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, L. delbrueckii subsp. lactis, L. helveticus, L. fermentum in whey starter for Grana Padano cheese. International Journal of Food Microbiology 146(2): 207-211.

  • Genini S, Badaoui B, Sclep G, Bishop SC, Waddington D, Pinard van der Laan MH, Klopp C, Cabau C, Seyfert HM, Petzl W, Jensen K, Glass EJ, de Greeff A, Smith HE, Smits MA, Olsaker I, Boman GM, Pisoni G, Moroni P, Castiglioni B, Cremonesi P, Del Corvo M, Foulon E, Foucras G, Rupp R, Giuffra E. (2011). Strengthening insights into host responses to mastitis infection in ruminants by combining heterogeneous microarray data sources. BMC Genomics, 12(1): 225.

  • Pisoni G, Moroni P, Genini S, Stella A, Boettcher P, Cremonesi P, Scaccabarozzi L, Giuffra E, Castiglioni B. (2010). Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats. Veterinary Immunology and Immunopathology 135 (3-4): 208-217.

  • Cremonesi P, Pisoni G, Severgnini M, Consolandi C, Moroni P, Raschetti M, Castiglioni B (2009). Pathogens detection in milk samples by LDR-mediated Universal Array method. Journal of Dairy Science 92: 3027–3039.

  • Severgnini M, Cremonesi P, Consolandi C, Caredda G, De Bellis G, Castiglioni B. (2009). ORMA: a tool for identification of species-specific variations in 16S rRNA gene and oligonucleotides design. Nucleic Acids Research, Vol.37, 1–15, doi:10.1093/nar/gkp499.

  • Cremonesi P, Perez G, Pisoni G, Castiglioni B, Luzzana M, Battaglia C, Sartorelli P, Moroni P. (2008). Development of a microarray platform for milk pathogens detection: preliminary research. Veterinary Research Communication, 32 (Suppl. 1): S187-S189.

  • Morandi S, Brasca M, Lodi R, Cremonesi P, Castiglioni B. (2007). Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and dairy products. Veterinary Microbiology 124: 66-72.

  • Cremonesi P, Perez G, Pisoni G, Moroni P, Morandi S, Luzzana M, Brasca M, Castiglioni B. (2007). Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. Letters in Applied Microbiology 45: 586-591.

  • Cremonesi P, Castiglioni B, Malferrari G, Biunno I, Vimercati C, Moroni P, Morandi S, Luzzana M. (2006). Technical Note: Improved method for rapid DNA extraction of mastitis pathogens directly from milk. Journal of Dairy Science 89: 163-169.

  • Cremonesi P, Vimercati C, Pisoni G, Castiglioni B, Luzzana M, Ruffo G, Moroni P. (2006). Identification of enterotoxin genes in Staphylococcus aureus isolates from bovine and caprine milk. Veterinary Research Communication 30 (Suppl. 1): 241-243.

  • Cremonesi P, Luzzana M, Brasca M, Morandi S, Lodi R, Vimercati C, Moroni P, Agnellini D, Caramenti G, Castiglioni B. (2005). Development of a multiplex polymerase chain reaction assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. Molecular and Cellular Probes 19: 299-305.

  • Moroni P, Pisoni G, Vimercati C, Rinaldi M, Castiglioni B, Cremonesi P, Boettcher P. (2005). Characterization of Staphylococcus aureus isolated from chronically infected dairy goats. Journal of Dairy Science 88 (10): 3500-3509.

Questa area è compilata sotto la responsabilità personale del titolare della pagina.

 
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