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BIANCA CASTIGLIONI

Bianca_in_lab_per_sito

Research Scientist

Email: castiglioni * ibba.cnr.it

Phone: +3903714662504

Fax: +3903714662501

Address: IBBA-CNR, U.O.S. di Lodi , Via Einstein, 26900 Lodi

 

Education and Professional Experience
1991: Degree in Biology at the University of Pavia, Italy.
1992-1996: Master in Applied Genetics at the University of Pavia, Italy.

1994-1998: Fellowship at the Italian National Research Council, Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, Milano.
1998-2000: Staff Scientist at the Italian National Research Council, Istituto per la Difesa e la Valorizzazione del Germoplasma Animale, Milano, within the UE Project: "Analysis of molecular factor affecting variability in BSE and scrapie susceptibility".
2000-2001: Staff Scientist at the  Italian National Research Council, Istituto di Tecnologie Biomediche, Segrate (Mi).
2001: Permanent position as Researcher at the Italian National Research Council, Istituto di Biologia e Biotecnologia Agraria, Lodi.

 

Keywords

Plant and Animal Biodiversity,Gene expression regulation,Traceability and safety of feed and food products

Animal genomics, Molecular biology, Molecular diagnostics of feed and foods, Biostatistics and bioinformatics

 

Ongoing projects

 

Participation time period: 07/2011- today

Progetto CISIA - Integrated Knowledge for Sustainability and Innovation of “Made in Italy” in the Agroalimentary System

Role: Scientific coordinator of the OR1.3.2“Genetic and functional characterization of autochthonous pig breeds in Southern Italy for the valorization of local products and for the conservation of biodiversity"

Involved organizations: CNR- ISPAAM, UNIVERSITÀ DEGLI STUDI DI CATANIA - DIPARTIMENTO D3A

 

Participation time period: 07/2013- today

Progetto FILAGRO – Strategie innovative e sostenibili per la filiera agroalimentare

Role: Scientific coordinator of the WP7 “Miglioramento della qualità delle produzioni lattiero-casearie lombarde””

Involved organizations: CNR – Regione Lombardia

 

Participation time period: 09/2013 – today

Progetto MIUR “SAFE & SMART" - Nuove tecnologie abilitanti per la food safety e l'integrità delle filiere agro-alimentari in uno scenario globale

Role: Scientific coordinator of the Task 1.1.5  “Studio finalizzato allo sviluppo di dispositivi per la determinazione simultanea di microrganismi patogeni in matrici alimentari (latte, carne e formaggio) basati su tecnologie RT-PCR”

Involved organizations: ENEA ; FONDAZIONE PARCO TECNOLOGICO PADANO ;GRANAROLO SPA , CNR-IBBR - SEZIONE DI PERUGIA ; TECNOALIMENTI ; UNIMI - DIPARTIMENTO DI SCIENZE PER GLI ALIMENTI, LA NUTRIZIONE E L'AMBIENTE (DEFENS)

 

Participation time period: 07/2012 – today

Progetto GENHOME – Resort tecnologico per la ricerca genomica applicata alle scienze animali

Role: Partecipant

Involved organizations: FONDAZIONE PARCO TECNOLOGICO PADANO, UNIVERSITA’ CATTOLICA DEL SACRO CUORE, UNIVERSITA’ DEGLI STUDI DI TERAMO , UNIVERSITA’ DEGLI STUDI DI SASSARI, UNIVERSITA’ DEGLI STUDI DI MILANO

 

Participation time period: 01/2012 – today

Progetto INTEROMICS - Sviluppo di una piattaforma integrata per l’applicazione delle scienze “omiche” alla definizione dei biomarcatori e profili diagnostici, predittivi, e teranostici

Role: Partecipant

Involved organizations: CNR-ITB, CNR- ISA, CNR-IASI, CNR-ICAR, CNR-IEIIT, CNR-IIT, CNR-IBFM, CNR-IAC, CNR-IMATI

 

 

Selected Publications

§ Capra E, Cremonesi P, Cortimiglia C, Bignoli G, Ricchi M, Moroni P, Pesce A, Luini M,Castiglioni B. Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank. (2014) Lett Appl Microbiol. 59(6):642-7.

§ Cremonesi P, Pisani LF, Lecchi C, Ceciliani F, Martino P, Bonastre AS, Karus A, Balzaretti C, Castiglioni B. Development of 23 individual TaqMan® real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions. (2014) Food Microbiol. 43:35-40.

§ Böhme K, Cremonesi P, Severgnini M, Villa TG, Fernández-No IC, Barros-Velázquez J,Castiglioni B, Calo-Mata P. Detection of food spoilage and pathogenic bacteria based on ligation detection reaction coupled to flow-through hybridization on membranes. (2014) Biomed Res Int. 2014:156323

§ Modesto P, Peletto S, Pisoni G, Cremonesi P, Castiglioni B, Colussi S, Caramelli M, Bronzo V, Moroni P, Acutis PL Evaluation of internal reference genes for quantitative expression analysis by real-time reverse transcription-PCR in somatic cells from goat milk.(2013) J Dairy Sci.;96(12):7932-44.

§ Cremonesi P., Chessa S.,Castiglioni B. Genome sequence and analysis of Lactobacillus helveticus. (2012) Front Microbiol, vol. 3, p. 435

§ Cremonesi P., Pozzi F., M. Ricchi M.,Castiglioni B., Luini M., Chessa S. Identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism. (2012) J Dairy Sci, vol. 95, p. 6963-8.

§ Cremonesi P., Capoferri R., Pisoni G., Del Corvo M., Strozzi F., Rupp R., Caillat H., Modesto P., Moroni P., Williams J.L.,Castiglioni B., Stella A. Response of the goat mammary gland to infection with Staphylococcus aureus revealed by gene expression profiling in milk somatic and white blood cells. (2012) BMC Genomics, vol. 13, p. 540-576

§ Böhme K, Morandi S, Cremonesi P, Fernández No I.C, Barros-Velázquez J, Castiglioni B, Brasca M, Cañas B, Calo-Mata P.  Characterization of Staphylococcus aureus strains isolated from Italian dairy products by MALDI-TOF mass fingerprinting. (2012)  ELECTROPHORESIS, vol. 33, p. 2355-2364

§ Cariani A., Piano A., Consolandi C., Severgnini M., Castiglioni B., Caredda G., Candela M., Serratore P., De Bellis G., Tinti F. Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach. (2012) INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol. 153, p. 474-482

§ Severgnini, M., Cremonesi, P., Consolandi, C., De Bellis, G., Castiglioni, B. Advances in DNA Microarray Technology for the Detection of Foodborne Pathogens. (2011) Food and Bioprocess Technology, 4 (6), pp. 936-953.

§ Lauri, A., Castiglioni, B., Mariani, P. Comprehensive analysis of Salmonella sequence polymorphisms and development of a LDR-UA assay for the detection and characterization of selected serotypes. (2011) Applied Microbiology and Biotechnology, 91 (1), pp. 189-210.

§ Genini, S., Badaoui, B., Sclep, G., Bishop, S.C., Waddington, D., Pinard van der Laan, M., Klopp, C., Cabau, C., Seyfert, H., Petzl, W., Jensen, K., Glass, E.J., de Greeff, A., Smith, H.E., Smits, M.A., Olsaker, I., Boman, G.M., Pisoni, G., Moroni, P., Castiglioni, B., Cremonesi, P., Del Corvo, M., Foulon, E., Foucras, G., Rupp, R., Giuffra, E. Strengthening insights into host responses to mastitis infection in ruminants by combining heterogeneous microarray data sources.(2011) BMC Genomics, 12, art. no. 225.

§ Gorni, C., Garino, C., Iacuaniello, S., Castiglioni, B., Stella, A., Restelli, G.L., Pagnacco, G., Mariani, P. Transcriptome analysis to identify differential gene expression affecting meat quality in heavy Italian pigs. (2011) Animal Genetics, 42 (2), pp. 161-171.

§ Cremonesi, P., Vanoni, L., Morandi, S., Silvetti, T., Castiglioni, B., Brasca, M. Development of a pentaplex PCR assay for the simultaneous detection of Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, L. delbrueckii subsp. lactis, L. helveticus, L. fermentum in whey starter for Grana Padano cheese. (2011) International Journal of Food Microbiology, 146 (2), pp. 207-211.

§ Lauri, A., Castiglioni, B., Morabito, S., Tozzoli, R., Consolandi, C., Mariani, P. A tool based on Ligation Detection Reaction-Universal Array (LDR-UA) for the characterization of VTEC by identification of virulence-associated and serogroup-specific genes. (2011) Molecular and Cellular Probes, 25 (1), pp. 35-43.

§ Lauri, A., Chessa, S., Raschetti, M., Castiglioni, B., Mariani, P., Caetano, A.R. New labelling technology for molecular probes applied to the ligation detection reaction-universal array system. (2011) Molecular Biotechnology, 47 (1), pp. 1-8.

§ Pisoni, G., Moroni, P., Genini, S., Stella, A., Boettcher, P.J., Cremonesi, P., Scaccabarozzi, L., Giuffra, E., Castiglioni, B. Differentially expressed genes associated with Staphylococcus aureus mastitis in dairy goats. (2010) Veterinary Immunology and Immunopathology, 135 (3-4), pp. 208-217.

§ Candela, M., Consolandi, C., Severgnini, M., Biagi, E., Castiglioni, B., Vitali, B., De Bellis, G., Brigidi, P. High taxonomic level fingerprint of the human intestinal microbiota by Ligase Detection Reaction - Universal Array approach. (2010) BMC Microbiology, 10, p. 116.

§ Conte, G., Mele, M., Chessa, S., Castiglioni, B., Serra, A., Pagnacco, G., Secchiari, P. Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle. (2010) Journal of Dairy Science, 93 (2), pp. 753-763.

§ Cristoni, S., Zingaro, L., Canton, C., Cremonesi, P., Castiglioni, B., Morandi, S., Brasca, M., Luzzana, M., Battaglia, C. Surface-activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A. (2009)Journal of Mass Spectrometry, 44 (10), pp. 1482-1488.

§ Cremonesi, P., Pisoni, G., Severgnini, M., Consolandi, C., Moroni, P., Raschetti, M., Castiglioni, B. Pathogen detection in milk samples by ligation detection reaction-mediated universal array method. (2009) Journal of Dairy Science, 92 (7), pp. 3027-3039..

§ Severgnini, M., Cremonesi, P., Consolandi, C., Caredda, G., De Bellis, G., Castiglioni, B. ORMA: A tool for identification of species-specific variations in 16S rRNA gene and oligonucleotides design. (2009) Nucleic Acids Research, 37 (16), pp. e109-e109.

§ Gorni, C., Iacuaniello, S., Castiglioni, B., Pagnacco, G., Mariani, P. Pig KALRN, MYH1, MLC2V, SNX13, AK1, and PPIA loci RH mapping and chromosome position refining. (2008) Genetics and Molecular Research, 7 (4), pp. 982-985.

§ Cremonesi, P., Perez, G., Pisoni, G., Moroni, P., Morandi, S., Luzzana, M., Brasca, M., Castiglioni, B. Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. (2007) Letters in Applied Microbiology, 45 (6), pp. 586-591.

§ Morandi, S., Brasca, M., Lodi, R., Cremonesi, P., Castiglioni, B. Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and dairy products. (2007) Veterinary Microbiology, 124 (1-2), pp. 66-72.

§ Mele, M., Conte, G., Castiglioni, B., Chessa, S., Macciotta, N.P.P., Serra, A., Buccioni, A., Pagnacco, G., Secchiari, P. Stearoyl-coenzyme a desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. (2007) Journal of Dairy Science, 90 (9), pp. 4458-4465.

§ Chessa, S., Chiatti, F., Ceriotti, G., Caroli, A., Consolandi, C., Pagnacco, G., Castiglioni, B. Development of a single nucleotide polymorphism genotyping microarray platform for the identification of bovine milk protein genetic polymorphisms. (2007) Journal of Dairy Science, 90 (1), pp. 451-464.

§ Vimercati, C., Cremonesi, P., Castiglioni, B., Pisoni, G., Boettcher, P.J., Stella, A.., Vicenzoni, G., Moroni, P. Molecular typing of Staphylococcus aureus isolated from cows, goats and sheep with intramammary infections on the basis of gene polymorphisms and toxins genes. (2006) Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 53 (9), pp. 423-428.

§ Cremonesi, P., Castiglioni, B., Malferrari, G., Biunno, I., Vimercati, C., Moroni, P., Morandi, S., Luzzana, M. Improved method for rapid DNA extraction of mastitis pathogens directly from milk. (2006) Journal of Dairy Science, 89 (1), pp. 163-169.

§ Cremonesi, P., Luzzana, M., Brasca, M., Morandi, S., Lodi, R., Vimercati, C., Agnellini, D., Caramenti, G., Moroni, P.,Castiglioni, B. Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. (2005) Molecular and Cellular Probes, 19 (5), pp. 299-305.

§ Castiglioni, B., Rizzi, E., Frosini, A., Sivonen, K., Rajaniemi, P., Rantala, A., Mugnai, M.A., Ventura, S., Wilmotte, A., Boutte, C., Grubisic, S., Balthasart, P., Consolandi, C., Bordoni, R., Mezzelani, A., Battaglia, C., De Bellis, G. Development of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of Cyanobacteria. (2004) Applied and Environmental Microbiology, 70 (12), pp. 7161-7172..

 

International Patent:PCT n. WO/2007/107862, International Application Number PCT/IB2007/000707: METHOD FOR THE IDENTIFICATION OF BOVINE MILK PROTEIN GENETIC POLYMORPHISMS. Inventors: Pagnacco G., Chessa S.,Castiglioni B.

 

International Ids

LinkedIn: pub/bianca-castiglioni/48/2a/123

Orcid ID: 0000-0003-2326-6701

Research Gate: profile/Bianca_Castiglioni

Scopus Author ID: 7003290977

WoS Researcher ID: G-9856-2013

 
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