• Full Screen
  • Wide Screen
  • Narrow Screen
  • Increase font size
  • Default font size
  • Decrease font size
  • EU e-Privacy Directive

    This website uses cookies to manage authentication, navigation, and other functions. By using our website, you agree that we can place these types of cookies on your device.

Animal Germoplasm Cryobank "Giuseppe Rognoni"- IBBA-CNR

Semen cryopreservation and cryobank storage are important tools for animal genetic resources preservation. These activities are complementary to in situ conservation strategy, in which animals are maintained within the environments or production systems in which they were developed. Material stored in a gene bank may serve as a backup that can be used if genetic problems occur in the live population. It becomes the preferred strategy when in situ conservation activities are not able to maintain the genetic variability and to avoid breed extinction. (FAO, 2012. Cryoconservation of animal genetic resources. FAO Animal Production and Health Guidelines No. 12. Rome).

The collection Animal Germoplasm Cryobank "Giuseppe Rognoni"- IBBA-CNR consists of biological samples collected from males of local breeds, cryopreserved as genetic reserve. Semen stored was collected with two different methods: by using an artificial vagina and through post mortem epididymal sperm collection. This last method, recently applied to farm animal cryobank setting-up, allows to overcome some practical difficulties as absence of trained males for semen collection on farm. In this context epididymal sperm extraction from testicles of slaughtered or castrated animals is the only option to collect gametes. Semen volume, sperm concentration, motility, viability and sperm morphology of each male were assessed after collection. Sperm were then packaged into 0.5 ml straws, by using a semi-automatic system of packaging. Then straws were frozen and stored in liquid nitrogen at -196°C.

The storage site of the Animal Germoplasm Cryobank "Giuseppe Rognoni"- IBBA-CNR has been created at the Institute of Agricultural Biology and Biotechnology at the Parco Tecnologico Padano (PTP) in Lodi. Particular attention was given to the safety both of personnel and genetic material stored. For this purpose the gene bank rooms are equipped with a oxygen saturation alarm system for the detection environmental oxygen saturation levels. Liquid nitrogen tanks are monitored by probes that, in case of a significant temperature variation, send through an alarm system signals to the gene bank manager. Information on donor animals, material stored and the localization of samples stored in the Animal Germoplasm Cryobank "Giuseppe Rognoni"- IBBA-CNR are managed through the specific software CryoWEB. The cryobank is a partner of the “Network of the Italian cryobanks of farm animal genetic resources” (www.genrescryonet.unimi.it).

Collection present in the Animal Germoplasm Cryobank "Giuseppe Rognoni"- IBBA-CNR:

1. Semen Bank of Italian local pig breeds.

2. Collection of the swine genetic type “Nero della Lomellina”.

3. Lombardia Farm Animal Genetic Resources Cryobank (LABank)

You are here